We inoculated EV seeds with two Pseudomonas species (P. koreensis A21, P. frederiksbergensis A176) isolated only from EV plants in the field and two Kocuria species found only in irAOC plants (K. palustris B56, K.marina D102, Figure S4). are common in nature as well as the normal skin flora and mucosa of humans and animals. Extraction, Isolation, Identification, and Testing of a Bacterial Specimen from the Wood Center Door Handle Alexander Gloger Introduction Entering into a public building is the same procedure for everybody: just grab the nov., isolated from the rhizoplane of the narrow-leaved cattail (Typha angustifolia). Occurrence of disinfectant-resistant bacteria in a fresh ... CASE REPORT The expanding spectrum of human infections caused by Kocuria species: a case report and literature review Shashikala Purty1,*, Rajagopalan Saranathan2,*, K Prashanth2, K Narayanan3, Johny Asir1, Chandrakesan Sheela Devi1 and Satish Kumar Amarnath4 Although not previously known to cause human infections, Kocuria species have now emerged as human pathogens, mostly in Does not produce a water-soluble exopigment. ca. Kocuria rhizophila DC2201. Isolation of the DNA was performed with a commercial DNA extraction kit (GenEJute rM Plant . morphology. Kocuria palustris was reported as another As-tolerant bacterium containing As-detoxifying genes indicating that such mechanisms may be common in genus Kocuria. SAR1 in crude oil biodegradation. and one Micrococcus sp., that have been identified after phylogenetic analysis as the strains Kocuria marina F-276,310, Kocuria palustris F-276,345, and Micrococcus yunnanensis F-256,446 . To request specific content that has not yet been migrated, please contact communications@asmusa.org. Introduction. Kocuria is one of the genera of actinobacteria that . Arbitrarily primed PCR analysis showed a clonal strain. Members of the genus Kocuria were isolated from a wide variety of natural . The environmental bacteria are known as the microorganisms that are able to survive in the outside environment with few of them (10%) are disease-causing while the other 90% are non-pathogenic. Kocuria is named after Miroslav Kocur, a Slovakian microbiologist. Title Kocuria palustris sp. Introduction. Sequence comparisons of the 16S rDNA indicated these isolates to be phylogenetic neighbours of members of the genus Kocuria, family Micrococcaceae, in which they represent two . 5-15 nm for gram . (HiMedia, India) as per the manufacturer instructions. Kocuria palustris Taxonomy Morphology Cultural characteristics Biochemical characters Ecology Pathogenicity References Phylum Actinobacteria, Class Actinobacteria, Order Micrococcales, Family Micrococcaceae, Genus Kocuria, Kocuria palustris Kovács et al. Environmental Isolate Case Files: Micrococcus luteus. The genus Kocuria was created from the genus Micrococcus on the basis of the phylogenetic and chemotaxonomic dissection of the genus Micrococcus. Microorganisms that typically form symptomless associations with internal plant tissues are termed endophytes. Alsammak 2 1 Department of General Science, College of Basic Education, University of Mosul, Mosul, Iraq. The isolates belonged to 16 genera and the relative frequency of actinomycetes did not differ . These species were selected among other specialists because they were more abundant at different . In the present study, proteolytic microorganisms from cured artisanal Nuodeng ham were investigated in order to identify and select potential starter cultures for its faster and safer fermentation. Endophytes associate with most plant species found in natural and managed ecosystems. 1995. The strain DDK6 formed a reddish-pink colony with a 2 to 3 mm diameter after two days of incubation at 30°C. The producer actinobacteria strains correspond to two Kocuria spp. A total of 76 putative actinomyces were isolated from the six medicinal plant species based on colony morphology. Staphylococcus and Salimicrobium accounted for 2% of the diversity at day 1, and then increased to 39% and 36%, respectively, at day 135. The Kocuria cultures were also used in lab either before or after we did a streak of our isolates. Capsules: Many strains are encapsulated and can be seen in India ink wet mounts. Solid waste dates (SWD) and corn steep liquor (CSL) as agro-industrial products were performed to enhance the performance of Kocuria sp. Some strains may form rough, wrinkled, matt, dry colonies. (Drechsler) Leonard & Suggs (1974) Macroscopic morphology Colonies on potato dextrose agar at 25°C are dark gray to olivaceous black with a black reverse. They are aerobic, but two species ( Table 2) are slightly facultatively anaerobic. a total of 23 endophytic bacteria with different colony morphologies were isolated, and they were identified based on the phylogeny of 16s rrna genes into 17 phylogenetic species within the genera bacillus (9 isolates in 4 species), kocuria (4 in 4), microbacterium (6 in 5), micrococcus (2 in 1), pseudomonas (1 isolate) and staphylococcus (1 … PubMed Article CAS Google Scholar nov. and Kocuria rhizophila sp. Extremophiles are organisms that live in extreme environments, as opposed to organisms that live in moderate (mesophilic) environments. nov., isolated from salt-fermented seafood Ji-Hyun Yun,1 Seong Woon Roh,1,2 Mi-Ja Jung,1 Min-Soo Kim,1,2 Eun-Jin Park,1 Kee-Sun Shin,2 Young-Do Nam1 and Jin-Woo Bae1 Correspondence Jin-Woo Bae baejw@khu.ac.kr 1Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea Identifying Corynebacterium in a pharmaceutical environment is quite common, due to their prevalence in range of environments. Endophytic bacteria are considered to originate from the external environment. 2 Department of Biology, College of Science, University of . The reference type strain Kocuria palustris strain TAGA 27 T MTCC 10490 T (=DSM 11925 T) was obtained from Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology, Chandigarh, India. During bacterial growth, high emulsifying Methods: Genotypic, morphological and physiological characteristics are used to describe two new species of Kocuria, for which we propose the names Kocuria palustris, type strain DSM 11925T and Kocuria. Kocuria belongs to the phylum Actinobacteria, class Actinobacteria, order Actinomycetales, sub order Micrococcinae and family Micrococcaceae. It is possible that Kocuria rhizophila came from the gym, or that it was a contaminant in my plate that was mistaken for a colony from the original swab. 2 Accordingly, a number of presumed sta-phylococcal pathologies might have been caused by Kocuria species, although it is plausible that a variety of presumed Kocuria infections might have actually been due . Its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including HRMS, extensive 1D and 2D NMR analysis, MS/MS fragmentation, and chemical degradation and Marfey's analysis of the resulting amino acid residues. Kocuria belongs to the phylum Actinobacteria, class Actinobacteria, order Actinomycetales, sub order Micrococcinae and family Micrococcaceae. Jing-Guo Xu, Jiao Meng, Wen-Jing Bao, Jia-Mu Kang, Jing-Yu Chen and Bei-Zhong Han * Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China. Nonencapsulated. Ars operon has been widely studied . Less commonly, it is responsible for complications including acute pyelonephritis, urethritis, epididymitis, and prostatitis. We herein report the first case of brain abscess caused by Kocuria varians, a gram-positive microorganism, in a 52-year-old man. ,.-----Kocuria palustris DSM 11925T (Y16263) Kocuria rosea DSM 204471 (X87756) 100 Kocuria polaris CMS 76or1 (AJ278868) colony morphology, including the appearance of colony, colony surfaces, elevation, edges, and colors. As a pharmaceutical microbiologist, you are probably no stranger to Micrococcus luteus since it is among the most commonly found organisms in pharmaceutical environmental monitoring. : #59536] Oxygen tolerance aerobe Journal Int. Rapid growth. Kocuria is a Gram-positive cocci arranged in pairs, short chains, tetrads, cubical packets of eight and irregular clusters. Produces light turbidity and a deposit in nutrient broth. It is of interest to note here that in this method, both, gram-positive (Kocuria palustris) and gram-negative (Sphingomonas) bacteria, are accessible to staining to practically the same extent, despite of the distinct chemical and morphological differences in their cell walls (e.g. Volume 49 Page(s) 167-173 Year 1999 They are mostly innocuous and widely distributed in nature in the microbiota of humans and animals. are aerobic, grow best at 30°C - 35°C, and are slow growing. Nap (1 g l-1) was added in ASW as sole carbon source as it has long been al. A previous literature search from 1995 to 2010 identified only 15 cases.6 Immunocompromised individuals are at greater risk. Cells were Gram-positive coccoid and formed no endospores. Isolation of two Kocuria species capable of growing on various polycyclic aromatic hydrocarbons Rifat Zubair Ahmed1*, Nuzhat Ahmed1 and Geoffrey Michael Gadd2 1Centre for Molecular Genetics, University of Karachi, Karachi 75270, Pakistan. The present research evaluated the effects of three probiotics on the growth performance and intestinal digestive enzyme activity in fingerlings of grass carp, Ctenopharyngodon idella.Three treatments (T‐1, T‐2 and T‐3) were fed with diets containing different viable bacteria with a final concentration 10 6 CFU g −1 feed (Bacillus coagulans, Rhodopseudomonas palustris and Lactobacillus . 47 Kocuria varians and Kocuria kristinae (Stackebrandt et al. nov., Isolated from Water Sample Collected from the River Brahmaputra, Assam, India Microbes that live anywhere in the human body and are not pathogenic to . Objective: The normal habitat of Kocuria palustris and K. rhizophila include mammalian skin, soil and rhizoplane. Int J Syst Bacteriol 49:167-173. Bacteriol. nov. and Kocuria rhizophila sp. Reclassification of strain CCM 132, previously classified as Kocuria varians, as Kocuria carniphila sp. Conidiophores may be up to 200 µm long, are septate, nonbranched, geniculate, and become pale near the apex. 1999; 49 :393-396. If you need support accessing content you are subscribed to, please contact customer service at 202-737-3600 or service@asmusa.org. Conidia are olivaceous brown . Kocuria is non-encapsulated, non-spore-forming and catalase-positive. 302 previously [7]. Infections related to Kocuria spp. Grows well at 22-37 ºC. Most of the isolates showed typical morphology of Streptomyces (Table S2, Supporting Information). Kocuria is a Gram-positive cocci arranged in pairs, short chains, tetrads, cubical packets of eight and irregular clusters. 14 Journal of Medical and Scientific Research CASe RePoRT Kocuria varians - An emerging cause of ocular infections Anita K videkar1,*, Pranathi B 1, Madhuri Gadde and Nashrah Nooreen1 1Department of Ophthalmology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad Abstract Purpose: We report a rare case of recurrent conjunctivitis caused by gram positive aerobic microorganism The main agents are K. kristinae, K. rosea, K. varians and K. palustris. palustris and K. rhizophila [2]. Two Gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (Typha angustifolia) collected from a floating mat in the Soroksár tributary of the Danube river, Hungary. It has been found in the milk of water deer and reindeer. Species description is based on a single isolate. Morphology: Rods. Kovacs G, Burghardt J, Pradella S, Schumann P, Stackebrandt E, Marialigeti K (1999) Kocuria palustris sp. Mycolic acids and teichoic acids are absent. Colonies are up to 4 mm in diameter, circular, slightly convex, smooth, and glistening. . Case presentation An 18 year old male was brought to a tertiary care hospital in Greater Noida in an Vol. Screening for growth on PAHs and respiration measurement Spores: No. Kocuria palustris − 18 − − . The soil actinomycete Kocuria rhizophila belongs to the suborder Micrococcineae, a divergent bacterial group for which only a limited amount of genomic information is currently available. To examine the hypothesis that rice (Oryza sativa, cultivar Kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. Mesophiles = 0, Extremophile = 1. M. D. Collins and others Table 1. Publication: Schumann P, Tindall BJ, Mendrock U, Kramer I, Stackebrandt E. Pelczaria aurantia ATCC 49321T (=DSM 12801T) is a strain of Kocuria rosea (Flugge 1886) Stackebrandt et al. A previous literature search from 1995 to 2010 identified only 15 cases.6 Immunocompromised individuals are at greater risk. Cells are coccoid, resembling Staphylococcus and Micrococcus, and can group in pairs, chains, tetrads, cubical arrangements of eight, or irregular clusters. Selected potential isolates were then identified by using Gram staining, spore staining, and biochemical tests consisting . Corynebacterium propinquum (C. propinquum) mainly colonizes the oropharyngeal region of the upper respiratory tract. 1999. Motility: Cells exhibit twitching motility due to presence of polar fimbrae. Colony morphology, cell morphology, motility and Gram's reaction of the strain were determined by using standard methods (Barrow . The dominant species Staph-ylococcus equorum, Salimicrobium salexigens, and Kocuria palustris accounted for 23.6%, 16.1%, and 10.9% of all isolates, respectively. Phenotypic identification by the automated bacterial system, Vitek compact II . The main agents are K. kristinae, K. rosea, K. varians and K. palustris. Kocuria kristinae is a facultative anaerobic, non-motile, gram positive coccus occurring in irregular clusters and tetrads. Kocuria, established by Stackebrandt et al., previously was classified into Micrococcus. (2000), this species is an earlier heterotypic synonym of Pelczaria aurantia Poston 1994. The recoverability of actinomycetes varied between different sponge sources; for example, Amphimedon aff. Members of the genus Kocuria represent coccoid Gram-positive, non-motile, non-spore forming bacteria of strictly aerobic metabolism and are members of the phylum Actinobacteria.The genus is relatively young and was separated from Micrococcus following extensive phylogenetic investigations in 1995 .To date, the genus Kocuria comprises around 20 species and is commonly encountered . are uncommon but can be opportunistic pathogens in immunocompromised patients with underlying diseases. are gram-positive, aerobic microorganisms and found as normal skin flora in humans and other mammals. morphology were observed microscopically as described . The yellow color of the colonies is similar to that of Kocuria rhizophila when streaked on a plate. @ref: 4540 BacDive-ID: 7643 DSM-Number: 11925 keywords: Bacteria, 16S sequence, genome sequence, aerobe, mesophilic description: Kocuria palustris TAGA27 is an aerobe, mesophilic bacterium that was isolated from rhizosphere of Typha angustiflora. Infection with Kocuria in humans is extremely rare. Kocuria spp have been reported to be normal flora of human skin and oral cavity and are usually regarded as laboratory contaminants and ignored when isolated in the clinical specimens undermining its pathogenic potential.Kocuria was first identified as a causative agent of urinary tract infection way back in 1974, and it was named as Micrococcus kristinae []. The structure herein . sequences of SAR1 with sequences obtained by doing a Blast searching revealed 96% similarity to Kocuria palustris strain TAGA27. It is a gram-positive coccus that can be found grouped together in a staphylococci "packet' formation. ence type strain Kocuria palustris strain TAGA 27T MTCC 10490T (=DSM 11925T) was obtained from Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology, Chan-digarh, India. Bacteriol. Staphylococcus saprophyticus is a Gram-positive, coagulase-negative, non-hemolytic coccus that is a common cause of uncomplicated urinary tract infections (UTIs), particularly in young sexually active females. Extremophiles are organisms that live in extreme environments, as opposed to organisms that live in moderate (mesophilic) environments. nov By L. Tvrzova Description of a Novel Actinobacterium Microbacterium assamensis sp. (2008) isolated Kocuria palustris from soil of a petrol pump and first time reported degradation of commercial diesel oil by K. palustris . A new thiazolyl peptide, kocurin (1), was isolated from culture broths of a marine-derived Kocuria palustris. Aim. HEBS1 had proximity to Brachybacterium paraconglomeratum, HEBS3 had proximity to Kocuria palustris, HEBS6 had . Kocuria species are Gram-positive bacteria members of the Micrococcus family. 1995), Kocuria palustris and Kocuria 48 rhizophila (Kovacs et al. Differential characteristics of isolate IFAM EL-30T and the following related Gram-positive species: Nesterenkonia halobia, Rothia mucilaginosa, Rothia dentocariosa, Rothia nasimurium, Kocuria kristinae, Kocuria varians, Kocuria rhizophila, Kocuria palustris and Kocuria rosea All of these bacteria can occur as cocci that may be clustered together. This bacterium is a Gram+coccus, catalase+, coagulase, and it is a common inhabitant of skin and oral mucosa. A case of persistent bloodstream infection with Kocuria rhizophila related to a damaged central venous catheter in a 3-year-old girl with Hirschsprung's disease is reported. Texture is woolly to cottony. Nonmotile. For anaerobiosis, the Data on mol% G+C, amino sugars in cell wall polysaccharide, menaquinone composition and fatty acid composition are given in Table 2. This category includes acidophiles, thermophiles, osmophiles, halophiles, oligotrophs, and others. Colonies are yellow. The strain is typically associated with human skin, particularly from the head, arms and legs; consequently . Kocuria rhizophila lives on mammalian skin and in soil (Savini et al). Size: 0.9 - 1.6 micrometers by 1.5 - 2.5 micrometers. Kocuria rhizophila is a coccoid, gram-positive bacterium that belongs to the family Micrococcaceae in the order Actinomycetales.The type strain of K. rhizophila (DSM 11926 T) was isolated from the rhizosphere of narrowleaf cattail (Typha angustifolia) ().The genus Kocuria was created from the genus Micrococcus on the basis of the phylogenetic and chemotaxonomic dissection of the genus . Costa Rica has a significant number of snakebites per year and bacterial infections are often complications in these animal bites. Microbes that live anywhere in the human body and are not pathogenic to . According to Schumann et al. Mm in diameter, circular, slightly convex, smooth, and non-spore forming to 200 µm long are! 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